pet-30a (+) expression vector (Millipore)
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pet-30a (+) expression vector
Pet 30a (+) Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet-30a+(+)+expression+vector/pet28a/10__1016_slash_j__pestbp__2024__106005-59-98-102
Average 90 stars, based on 1 article reviews
Pet 30a (+) Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet-30a+(+)+expression+vector/pet28a/10__1016_slash_j__pestbp__2024__106005-59-98-102
Average 90 stars, based on 1 article reviews
pet-30a (+) expression vector - by Bioz Stars,
2026-11
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Expressing:Article Title: A novel caspase-associated recruitment domain (CARD) containing protein (CgCARDCP-1) involved in LPS recognition and NF-κB activation in oyster (Crassostrea gigas). Article Snippet: AC CE Caspase-associated recruitment domain (CARD) containing proteins play 18 critical roles in molecular interaction and regulation of various signaling pathways, 19 such as the activation of caspase and NF-κB singling pathway in the process of 20 apoptosis or inflammation.. In the present study, a novel CARD containing protein 21 (designed CgCARDCP-1) was identified and characterized from oyster Crassostrea 22 M AN US CR IP T AC CE PT ED gigas.. Molecular feature analysis revealed that, the open reading frame (ORF) of 23 CgCARDCP-1 gene was 759 bp encoding a polypeptide of 253 amino acids with a 24 conserved N-terminal CARD domain and two transcriptional coactivator p15 (PC4) 25 domains in C-terminus. Article Title: Molecular identification and functional analysis of a tumor necrosis factor superfamily gene from Chinese mitten crab (Eriocheir sinensis). Article Snippet: Tumor necrosis factor (TNF) is one of the most important cytokines involved in various biological processes in vertebrates and invertebrates.. In the present study, a new member of the TNF superfamily (named EsTNFSF) was identified from the Chinese mitten crab (Eriocheir sinensis).. The full-length cDNA of EsTNFSF is 2462 bp and encodes a polypeptide with 499 amino acids. Article Title: Molecular characterization of seven novel Glu-A1mx alleles from Triticum monococcum ssp. monococcum Article Snippet: The resulting fragments were cloned into the Article Title: Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas Article Snippet: The PCR product of the cathepsin L cDNA was gel-purified and cloned into a Article Title: A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice Article Snippet: 23 Briefly, the Article Title: Antiviral activity of porcine interferon delta 8 against foot-and-mouth disease virus in vitro. Article Snippet: The vector containing the target gene flanked by EcoRI and Hind III restriction sites, was digested with the corresponding enzymes and ligated into the same sites in a pET-30a expression vector (Novagen, Corporation, CA, USA) to generate pET30a-PoIFN-δ8. Article Title: Comparative study of three C1q domain containing proteins from pacific oyster Crassostrea gigas. Article Snippet: C1q domain containing proteins (C1qDCs) are a family of proteins containing a globular head C1q domain (ghC1q) in C-terminus, which serve as pattern recognition receptors (PRRs) and mediate a series of immune responses.. In the present study, three C1qDC proteins from pacific oyster Crassostrea gigas (CgC1qDC-2, CgC1qDC-3, CgC1qDC-4) were characterized and comparatively investigated to understand their roles in the immune response.. All the three recombinant CgC1qDC proteins (rCgC1qDCs) could bind lipopolysaccharide (LPS) significantly but they could not bind lipoteichoic acid (LTA), b-1,3-glucan (GLU), mannan (MAN), and polyinosinic-polycytidylic acid (Poly I:C). Article Title: LcMYB4 , an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis Article Snippet: To obtain the protein interacting with LcMYB4, the ORF sequence of LcMYB4 was cloned into the pET-30a expression vector (Novagen, Madison, WI) to produce His 6 tag-fused LcMYB4. Plasmid Preparation:Article Title: A novel caspase-associated recruitment domain (CARD) containing protein (CgCARDCP-1) involved in LPS recognition and NF-κB activation in oyster (Crassostrea gigas). Article Snippet: AC CE Caspase-associated recruitment domain (CARD) containing proteins play 18 critical roles in molecular interaction and regulation of various signaling pathways, 19 such as the activation of caspase and NF-κB singling pathway in the process of 20 apoptosis or inflammation.. In the present study, a novel CARD containing protein 21 (designed CgCARDCP-1) was identified and characterized from oyster Crassostrea 22 M AN US CR IP T AC CE PT ED gigas.. Molecular feature analysis revealed that, the open reading frame (ORF) of 23 CgCARDCP-1 gene was 759 bp encoding a polypeptide of 253 amino acids with a 24 conserved N-terminal CARD domain and two transcriptional coactivator p15 (PC4) 25 domains in C-terminus. Article Title: Molecular identification and functional analysis of a tumor necrosis factor superfamily gene from Chinese mitten crab (Eriocheir sinensis). Article Snippet: Tumor necrosis factor (TNF) is one of the most important cytokines involved in various biological processes in vertebrates and invertebrates.. In the present study, a new member of the TNF superfamily (named EsTNFSF) was identified from the Chinese mitten crab (Eriocheir sinensis).. The full-length cDNA of EsTNFSF is 2462 bp and encodes a polypeptide with 499 amino acids. Article Title: Molecular characterization of seven novel Glu-A1mx alleles from Triticum monococcum ssp. monococcum Article Snippet: The resulting fragments were cloned into the Article Title: Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas Article Snippet: The PCR product of the cathepsin L cDNA was gel-purified and cloned into a Article Title: A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice Article Snippet: 23 Briefly, the Article Title: Antiviral activity of porcine interferon delta 8 against foot-and-mouth disease virus in vitro. Article Snippet: The vector containing the target gene flanked by EcoRI and Hind III restriction sites, was digested with the corresponding enzymes and ligated into the same sites in a pET-30a expression vector (Novagen, Corporation, CA, USA) to generate pET30a-PoIFN-δ8. Article Title: Comparative study of three C1q domain containing proteins from pacific oyster Crassostrea gigas. Article Snippet: C1q domain containing proteins (C1qDCs) are a family of proteins containing a globular head C1q domain (ghC1q) in C-terminus, which serve as pattern recognition receptors (PRRs) and mediate a series of immune responses.. In the present study, three C1qDC proteins from pacific oyster Crassostrea gigas (CgC1qDC-2, CgC1qDC-3, CgC1qDC-4) were characterized and comparatively investigated to understand their roles in the immune response.. All the three recombinant CgC1qDC proteins (rCgC1qDCs) could bind lipopolysaccharide (LPS) significantly but they could not bind lipoteichoic acid (LTA), b-1,3-glucan (GLU), mannan (MAN), and polyinosinic-polycytidylic acid (Poly I:C). Article Title: LcMYB4 , an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis Article Snippet: To obtain the protein interacting with LcMYB4, the ORF sequence of LcMYB4 was cloned into the pET-30a expression vector (Novagen, Madison, WI) to produce His 6 tag-fused LcMYB4. Sequencing:Article Title: A novel caspase-associated recruitment domain (CARD) containing protein (CgCARDCP-1) involved in LPS recognition and NF-κB activation in oyster (Crassostrea gigas). Article Snippet: AC CE Caspase-associated recruitment domain (CARD) containing proteins play 18 critical roles in molecular interaction and regulation of various signaling pathways, 19 such as the activation of caspase and NF-κB singling pathway in the process of 20 apoptosis or inflammation.. In the present study, a novel CARD containing protein 21 (designed CgCARDCP-1) was identified and characterized from oyster Crassostrea 22 M AN US CR IP T AC CE PT ED gigas.. Molecular feature analysis revealed that, the open reading frame (ORF) of 23 CgCARDCP-1 gene was 759 bp encoding a polypeptide of 253 amino acids with a 24 conserved N-terminal CARD domain and two transcriptional coactivator p15 (PC4) 25 domains in C-terminus. Article Title: Molecular identification and functional analysis of a tumor necrosis factor superfamily gene from Chinese mitten crab (Eriocheir sinensis). Article Snippet: Tumor necrosis factor (TNF) is one of the most important cytokines involved in various biological processes in vertebrates and invertebrates.. In the present study, a new member of the TNF superfamily (named EsTNFSF) was identified from the Chinese mitten crab (Eriocheir sinensis).. The full-length cDNA of EsTNFSF is 2462 bp and encodes a polypeptide with 499 amino acids. Article Title: Molecular characterization of seven novel Glu-A1mx alleles from Triticum monococcum ssp. monococcum Article Snippet: The resulting fragments were cloned into the Article Title: Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas Article Snippet: The PCR product of the cathepsin L cDNA was gel-purified and cloned into a Article Title: A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice Article Snippet: 23 Briefly, the Article Title: Antiviral activity of porcine interferon delta 8 against foot-and-mouth disease virus in vitro. Article Snippet: The vector containing the target gene flanked by EcoRI and Hind III restriction sites, was digested with the corresponding enzymes and ligated into the same sites in a pET-30a expression vector (Novagen, Corporation, CA, USA) to generate pET30a-PoIFN-δ8. Article Title: Comparative study of three C1q domain containing proteins from pacific oyster Crassostrea gigas. Article Snippet: C1q domain containing proteins (C1qDCs) are a family of proteins containing a globular head C1q domain (ghC1q) in C-terminus, which serve as pattern recognition receptors (PRRs) and mediate a series of immune responses.. In the present study, three C1qDC proteins from pacific oyster Crassostrea gigas (CgC1qDC-2, CgC1qDC-3, CgC1qDC-4) were characterized and comparatively investigated to understand their roles in the immune response.. All the three recombinant CgC1qDC proteins (rCgC1qDCs) could bind lipopolysaccharide (LPS) significantly but they could not bind lipoteichoic acid (LTA), b-1,3-glucan (GLU), mannan (MAN), and polyinosinic-polycytidylic acid (Poly I:C). Article Title: LcMYB4 , an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis Article Snippet: To obtain the protein interacting with LcMYB4, the ORF sequence of LcMYB4 was cloned into the pET-30a expression vector (Novagen, Madison, WI) to produce His 6 tag-fused LcMYB4. Clone Assay:Article Title: A novel caspase-associated recruitment domain (CARD) containing protein (CgCARDCP-1) involved in LPS recognition and NF-κB activation in oyster (Crassostrea gigas). Article Snippet: AC CE Caspase-associated recruitment domain (CARD) containing proteins play 18 critical roles in molecular interaction and regulation of various signaling pathways, 19 such as the activation of caspase and NF-κB singling pathway in the process of 20 apoptosis or inflammation.. In the present study, a novel CARD containing protein 21 (designed CgCARDCP-1) was identified and characterized from oyster Crassostrea 22 M AN US CR IP T AC CE PT ED gigas.. Molecular feature analysis revealed that, the open reading frame (ORF) of 23 CgCARDCP-1 gene was 759 bp encoding a polypeptide of 253 amino acids with a 24 conserved N-terminal CARD domain and two transcriptional coactivator p15 (PC4) 25 domains in C-terminus. Article Title: Molecular identification and functional analysis of a tumor necrosis factor superfamily gene from Chinese mitten crab (Eriocheir sinensis). Article Snippet: Tumor necrosis factor (TNF) is one of the most important cytokines involved in various biological processes in vertebrates and invertebrates.. In the present study, a new member of the TNF superfamily (named EsTNFSF) was identified from the Chinese mitten crab (Eriocheir sinensis).. The full-length cDNA of EsTNFSF is 2462 bp and encodes a polypeptide with 499 amino acids. Article Title: Molecular characterization of seven novel Glu-A1mx alleles from Triticum monococcum ssp. monococcum Article Snippet: The resulting fragments were cloned into the Article Title: Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas Article Snippet: The PCR product of the cathepsin L cDNA was gel-purified and cloned into a Article Title: A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice Article Snippet: 23 Briefly, the Article Title: Antiviral activity of porcine interferon delta 8 against foot-and-mouth disease virus in vitro. Article Snippet: The vector containing the target gene flanked by EcoRI and Hind III restriction sites, was digested with the corresponding enzymes and ligated into the same sites in a pET-30a expression vector (Novagen, Corporation, CA, USA) to generate pET30a-PoIFN-δ8. Article Title: Comparative study of three C1q domain containing proteins from pacific oyster Crassostrea gigas. Article Snippet: C1q domain containing proteins (C1qDCs) are a family of proteins containing a globular head C1q domain (ghC1q) in C-terminus, which serve as pattern recognition receptors (PRRs) and mediate a series of immune responses.. In the present study, three C1qDC proteins from pacific oyster Crassostrea gigas (CgC1qDC-2, CgC1qDC-3, CgC1qDC-4) were characterized and comparatively investigated to understand their roles in the immune response.. All the three recombinant CgC1qDC proteins (rCgC1qDCs) could bind lipopolysaccharide (LPS) significantly but they could not bind lipoteichoic acid (LTA), b-1,3-glucan (GLU), mannan (MAN), and polyinosinic-polycytidylic acid (Poly I:C). Article Title: LcMYB4 , an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis Article Snippet: To obtain the protein interacting with LcMYB4, the ORF sequence of LcMYB4 was cloned into the pET-30a expression vector (Novagen, Madison, WI) to produce His 6 tag-fused LcMYB4. Positron Emission Tomography:Article Title: A novel caspase-associated recruitment domain (CARD) containing protein (CgCARDCP-1) involved in LPS recognition and NF-κB activation in oyster (Crassostrea gigas). Article Snippet: AC CE Caspase-associated recruitment domain (CARD) containing proteins play 18 critical roles in molecular interaction and regulation of various signaling pathways, 19 such as the activation of caspase and NF-κB singling pathway in the process of 20 apoptosis or inflammation.. In the present study, a novel CARD containing protein 21 (designed CgCARDCP-1) was identified and characterized from oyster Crassostrea 22 M AN US CR IP T AC CE PT ED gigas.. Molecular feature analysis revealed that, the open reading frame (ORF) of 23 CgCARDCP-1 gene was 759 bp encoding a polypeptide of 253 amino acids with a 24 conserved N-terminal CARD domain and two transcriptional coactivator p15 (PC4) 25 domains in C-terminus. Article Title: Molecular identification and functional analysis of a tumor necrosis factor superfamily gene from Chinese mitten crab (Eriocheir sinensis). Article Snippet: Tumor necrosis factor (TNF) is one of the most important cytokines involved in various biological processes in vertebrates and invertebrates.. In the present study, a new member of the TNF superfamily (named EsTNFSF) was identified from the Chinese mitten crab (Eriocheir sinensis).. The full-length cDNA of EsTNFSF is 2462 bp and encodes a polypeptide with 499 amino acids. Article Title: Molecular characterization of seven novel Glu-A1mx alleles from Triticum monococcum ssp. monococcum Article Snippet: The resulting fragments were cloned into the Article Title: Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas Article Snippet: The PCR product of the cathepsin L cDNA was gel-purified and cloned into a Article Title: A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice Article Snippet: 23 Briefly, the Article Title: Antiviral activity of porcine interferon delta 8 against foot-and-mouth disease virus in vitro. Article Snippet: The vector containing the target gene flanked by EcoRI and Hind III restriction sites, was digested with the corresponding enzymes and ligated into the same sites in a pET-30a expression vector (Novagen, Corporation, CA, USA) to generate pET30a-PoIFN-δ8. Article Title: Comparative study of three C1q domain containing proteins from pacific oyster Crassostrea gigas. Article Snippet: C1q domain containing proteins (C1qDCs) are a family of proteins containing a globular head C1q domain (ghC1q) in C-terminus, which serve as pattern recognition receptors (PRRs) and mediate a series of immune responses.. In the present study, three C1qDC proteins from pacific oyster Crassostrea gigas (CgC1qDC-2, CgC1qDC-3, CgC1qDC-4) were characterized and comparatively investigated to understand their roles in the immune response.. All the three recombinant CgC1qDC proteins (rCgC1qDCs) could bind lipopolysaccharide (LPS) significantly but they could not bind lipoteichoic acid (LTA), b-1,3-glucan (GLU), mannan (MAN), and polyinosinic-polycytidylic acid (Poly I:C). Article Title: LcMYB4 , an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis Article Snippet: To obtain the protein interacting with LcMYB4, the ORF sequence of LcMYB4 was cloned into the pET-30a expression vector (Novagen, Madison, WI) to produce His 6 tag-fused LcMYB4. Purification:Article Title: A novel caspase-associated recruitment domain (CARD) containing protein (CgCARDCP-1) involved in LPS recognition and NF-κB activation in oyster (Crassostrea gigas). Article Snippet: AC CE Caspase-associated recruitment domain (CARD) containing proteins play 18 critical roles in molecular interaction and regulation of various signaling pathways, 19 such as the activation of caspase and NF-κB singling pathway in the process of 20 apoptosis or inflammation.. In the present study, a novel CARD containing protein 21 (designed CgCARDCP-1) was identified and characterized from oyster Crassostrea 22 M AN US CR IP T AC CE PT ED gigas.. Molecular feature analysis revealed that, the open reading frame (ORF) of 23 CgCARDCP-1 gene was 759 bp encoding a polypeptide of 253 amino acids with a 24 conserved N-terminal CARD domain and two transcriptional coactivator p15 (PC4) 25 domains in C-terminus. Article Title: Molecular identification and functional analysis of a tumor necrosis factor superfamily gene from Chinese mitten crab (Eriocheir sinensis). Article Snippet: Tumor necrosis factor (TNF) is one of the most important cytokines involved in various biological processes in vertebrates and invertebrates.. In the present study, a new member of the TNF superfamily (named EsTNFSF) was identified from the Chinese mitten crab (Eriocheir sinensis).. The full-length cDNA of EsTNFSF is 2462 bp and encodes a polypeptide with 499 amino acids. Article Title: Molecular characterization of seven novel Glu-A1mx alleles from Triticum monococcum ssp. monococcum Article Snippet: The resulting fragments were cloned into the Article Title: Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas Article Snippet: The PCR product of the cathepsin L cDNA was gel-purified and cloned into a Article Title: A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice Article Snippet: 23 Briefly, the Article Title: Antiviral activity of porcine interferon delta 8 against foot-and-mouth disease virus in vitro. Article Snippet: The vector containing the target gene flanked by EcoRI and Hind III restriction sites, was digested with the corresponding enzymes and ligated into the same sites in a pET-30a expression vector (Novagen, Corporation, CA, USA) to generate pET30a-PoIFN-δ8. Article Title: Comparative study of three C1q domain containing proteins from pacific oyster Crassostrea gigas. Article Snippet: C1q domain containing proteins (C1qDCs) are a family of proteins containing a globular head C1q domain (ghC1q) in C-terminus, which serve as pattern recognition receptors (PRRs) and mediate a series of immune responses.. In the present study, three C1qDC proteins from pacific oyster Crassostrea gigas (CgC1qDC-2, CgC1qDC-3, CgC1qDC-4) were characterized and comparatively investigated to understand their roles in the immune response.. All the three recombinant CgC1qDC proteins (rCgC1qDCs) could bind lipopolysaccharide (LPS) significantly but they could not bind lipoteichoic acid (LTA), b-1,3-glucan (GLU), mannan (MAN), and polyinosinic-polycytidylic acid (Poly I:C). Article Title: LcMYB4 , an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis Article Snippet: To obtain the protein interacting with LcMYB4, the ORF sequence of LcMYB4 was cloned into the pET-30a expression vector (Novagen, Madison, WI) to produce His 6 tag-fused LcMYB4. Recombinant:Article Title: A novel caspase-associated recruitment domain (CARD) containing protein (CgCARDCP-1) involved in LPS recognition and NF-κB activation in oyster (Crassostrea gigas). Article Snippet: AC CE Caspase-associated recruitment domain (CARD) containing proteins play 18 critical roles in molecular interaction and regulation of various signaling pathways, 19 such as the activation of caspase and NF-κB singling pathway in the process of 20 apoptosis or inflammation.. In the present study, a novel CARD containing protein 21 (designed CgCARDCP-1) was identified and characterized from oyster Crassostrea 22 M AN US CR IP T AC CE PT ED gigas.. Molecular feature analysis revealed that, the open reading frame (ORF) of 23 CgCARDCP-1 gene was 759 bp encoding a polypeptide of 253 amino acids with a 24 conserved N-terminal CARD domain and two transcriptional coactivator p15 (PC4) 25 domains in C-terminus. Article Title: Molecular identification and functional analysis of a tumor necrosis factor superfamily gene from Chinese mitten crab (Eriocheir sinensis). Article Snippet: Tumor necrosis factor (TNF) is one of the most important cytokines involved in various biological processes in vertebrates and invertebrates.. In the present study, a new member of the TNF superfamily (named EsTNFSF) was identified from the Chinese mitten crab (Eriocheir sinensis).. The full-length cDNA of EsTNFSF is 2462 bp and encodes a polypeptide with 499 amino acids. Article Title: Molecular characterization of seven novel Glu-A1mx alleles from Triticum monococcum ssp. monococcum Article Snippet: The resulting fragments were cloned into the Article Title: Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas Article Snippet: The PCR product of the cathepsin L cDNA was gel-purified and cloned into a Article Title: A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice Article Snippet: 23 Briefly, the Article Title: Antiviral activity of porcine interferon delta 8 against foot-and-mouth disease virus in vitro. Article Snippet: The vector containing the target gene flanked by EcoRI and Hind III restriction sites, was digested with the corresponding enzymes and ligated into the same sites in a pET-30a expression vector (Novagen, Corporation, CA, USA) to generate pET30a-PoIFN-δ8. Article Title: Comparative study of three C1q domain containing proteins from pacific oyster Crassostrea gigas. Article Snippet: C1q domain containing proteins (C1qDCs) are a family of proteins containing a globular head C1q domain (ghC1q) in C-terminus, which serve as pattern recognition receptors (PRRs) and mediate a series of immune responses.. In the present study, three C1qDC proteins from pacific oyster Crassostrea gigas (CgC1qDC-2, CgC1qDC-3, CgC1qDC-4) were characterized and comparatively investigated to understand their roles in the immune response.. All the three recombinant CgC1qDC proteins (rCgC1qDCs) could bind lipopolysaccharide (LPS) significantly but they could not bind lipoteichoic acid (LTA), b-1,3-glucan (GLU), mannan (MAN), and polyinosinic-polycytidylic acid (Poly I:C). Article Title: LcMYB4 , an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis Article Snippet: To obtain the protein interacting with LcMYB4, the ORF sequence of LcMYB4 was cloned into the pET-30a expression vector (Novagen, Madison, WI) to produce His 6 tag-fused LcMYB4. Polymerase Chain Reaction:Article Title: A novel caspase-associated recruitment domain (CARD) containing protein (CgCARDCP-1) involved in LPS recognition and NF-κB activation in oyster (Crassostrea gigas). Article Snippet: AC CE Caspase-associated recruitment domain (CARD) containing proteins play 18 critical roles in molecular interaction and regulation of various signaling pathways, 19 such as the activation of caspase and NF-κB singling pathway in the process of 20 apoptosis or inflammation.. In the present study, a novel CARD containing protein 21 (designed CgCARDCP-1) was identified and characterized from oyster Crassostrea 22 M AN US CR IP T AC CE PT ED gigas.. Molecular feature analysis revealed that, the open reading frame (ORF) of 23 CgCARDCP-1 gene was 759 bp encoding a polypeptide of 253 amino acids with a 24 conserved N-terminal CARD domain and two transcriptional coactivator p15 (PC4) 25 domains in C-terminus. Article Title: Molecular identification and functional analysis of a tumor necrosis factor superfamily gene from Chinese mitten crab (Eriocheir sinensis). Article Snippet: Tumor necrosis factor (TNF) is one of the most important cytokines involved in various biological processes in vertebrates and invertebrates.. In the present study, a new member of the TNF superfamily (named EsTNFSF) was identified from the Chinese mitten crab (Eriocheir sinensis).. The full-length cDNA of EsTNFSF is 2462 bp and encodes a polypeptide with 499 amino acids. Article Title: Molecular characterization of seven novel Glu-A1mx alleles from Triticum monococcum ssp. monococcum Article Snippet: The resulting fragments were cloned into the Article Title: Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas Article Snippet: The PCR product of the cathepsin L cDNA was gel-purified and cloned into a Article Title: A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice Article Snippet: 23 Briefly, the Article Title: Antiviral activity of porcine interferon delta 8 against foot-and-mouth disease virus in vitro. Article Snippet: The vector containing the target gene flanked by EcoRI and Hind III restriction sites, was digested with the corresponding enzymes and ligated into the same sites in a pET-30a expression vector (Novagen, Corporation, CA, USA) to generate pET30a-PoIFN-δ8. Article Title: Comparative study of three C1q domain containing proteins from pacific oyster Crassostrea gigas. Article Snippet: C1q domain containing proteins (C1qDCs) are a family of proteins containing a globular head C1q domain (ghC1q) in C-terminus, which serve as pattern recognition receptors (PRRs) and mediate a series of immune responses.. In the present study, three C1qDC proteins from pacific oyster Crassostrea gigas (CgC1qDC-2, CgC1qDC-3, CgC1qDC-4) were characterized and comparatively investigated to understand their roles in the immune response.. All the three recombinant CgC1qDC proteins (rCgC1qDCs) could bind lipopolysaccharide (LPS) significantly but they could not bind lipoteichoic acid (LTA), b-1,3-glucan (GLU), mannan (MAN), and polyinosinic-polycytidylic acid (Poly I:C). Article Title: LcMYB4 , an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis Article Snippet: To obtain the protein interacting with LcMYB4, the ORF sequence of LcMYB4 was cloned into the pET-30a expression vector (Novagen, Madison, WI) to produce His 6 tag-fused LcMYB4. |